Spin with virus at 1000g 120 min at 37C (32*C for retrovirus). Return to incubator. After 2 hours aspirate media and replace with fresh virus-free media. Treat any unused diluted virus with 10% bleach for 45 minutes before discarding into a biohazard waste container. Retroviruses are potent disease agents, but they have also served as invaluable research tools in molecular biology. In 1979, the molecular biologist Richard Mulligan used a genetically altered retrovirus to trigger the production of hemoglobin in vitro by monkey kidney cells.
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